Fernanda cisneros

Handling Editor: David Glover. Nuno M. Earnshaw; H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory. J Cell Fernanda cisneros 15 July ; 14 : jcs

Background: In cancer cells, transcriptional gene silencing has been associated with genetic and epigenetic defects. The disruption of DNA methylation patterns and covalent histone marks has been associated with cancer development. In particular, miRb1 has been suggested to be an miRNA with tumor suppressor activity, and it has been shown to be deregulated in various human cancers. In the present study, we evaluated the DNA methylation at the CpG island proximal to the transcription start site of miRb1 in cancer cell lines as well as in normal tissues and gynecological tumor samples. In addition, we analyzed the association of CTCF and covalent histone modifications at the miRb1 locus.

Fernanda cisneros

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Our understanding of the structure and function of mitotic chromosomes has come a long way since these iconic objects were first recognized more than years ago, though many details remain to be elucidated. In this chapter, we start with the early history of chromosome studies and then describe the path that led to our current understanding of the formation and structure of mitotic chromosomes. We also discuss some of the remaining questions. It is now well established that each mitotic chromatid consists of a central organizing region containing a so-called "chromosome scaffold" from which loops of DNA project radially. These proteins are concentrated along the axis of the chromatid.

Fernanda cisneros

J Cell Sci 15 July ; 14 : jcs First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Nuno conducted the research described in this article while a PhD student in William C. He is now a postdoc in the lab of Ting Wu at Harvard Medical School, Boston, USA, where his research interests lie in the structural and dynamic chromatin regulation of the more mysterious regions of the cell nucleus, such as centromeres, repetitive elements and nucleoli. Fernanda conducted the research described in this article while a postdoc in William C. Earnshaw's lab. Earnshaw and is interested in developing human artificial chromosomes HACs by applying molecular and synthetic biology techniques to study chromosome segregation and epigenetics in human cells. Our work, which manipulated an artificial chromosome inserted in human cells so the cell's normal chromosomes could survive this process , showed that the centromere remains in place and still works, even when the mechanisms that regulate the jungle are picked apart.

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Sign in. J Cell Sci 14 : jcs Vladimir Larionov. H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory In collection: Exploring the Nucleus. Article history Received:. In addition, we analyzed the association of CTCF and covalent histone modifications at the miRb1 locus. Japan Society for the Promotion of Science 17J Search Dropdown Menu. Tetsuya Hori , Tetsuya Hori. Elisa Pesenti Wei-Hao Shang. Sign In or Create an Account. In the present study, we evaluated the DNA methylation at the CpG island proximal to the transcription start site of miRb1 in cancer cell lines as well as in normal tissues and gynecological tumor samples. Twitter LinkedIn Youtube.

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Latest published articles alert. Published by The Company of Biologists Ltd. Table of contents alert. Vladimir Larionov. H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory In collection: Exploring the Nucleus. Twitter LinkedIn Youtube. Japan Society for the Promotion of Science 17J Fernanda Cisneros-Soberanis Skip Nav Destination Close navigation menu Article navigation. Accepted manuscripts alert. Tatsuo Fukagawa. Tatsuo Fukagawa In order to investigate the importance of heterochromatin at centromeres, we used epigenetic engineering of a synthetic alphoid tetO human artificial chromosome HAC , to which chimeric proteins can be targeted. Sign in.