Proc natl acad sci u s a

Abstract : A key component of scientific communication is sufficient information for other researchers in the field to reproduce published findings, proc natl acad sci u s a. For computational and data-enabled research, this has often been interpreted to mean making available the raw data from which results were generated, the computer code that generated the findings, and any additional information needed such as workflows and input parameters. Many journals are revising author guidelines to include data and code availability. This work evaluates the effectiveness of journal policy that requires the data and code necessary for reproducibility be made available postpublication by the authors upon request.

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Proc natl acad sci u s a

A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was obtained with no loss of resolution, but the transfer was not quantitative. The method allows detection of proteins by autoradiography and is simpler than conventional procedures. The immobilized proteins were detectable by immunological procedures. All additional binding capacity on the nitrocellulose was blocked with excess protein; then a specific antibody was bound and, finally, a second antibody directed against the first antibody. The second antibody was either radioactively labeled or conjugated to fluorescein or to peroxidase. The specific protein was then detected by either autoradiography, under UV light, or by the peroxidase reaction product, respectively. In the latter case, as little as pg of protein was clearly detectable. It is anticipated that the procedure will be applicable to analysis of a wide variety of proteins with specific reactions or ligands. Abstract A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets.

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Natural genes and proteins often contain tandemly repeated sequence motifs that dramatically increase physiological specificity and activity. Given the selective value of such repeats, it is likely that several different mechanisms have been responsible for their generation. One mechanism that has been shown to generate relatively long tandem repeats in the kilobase range is rolling circle replication. In this communication, we demonstrate that rolling circle synthesis in a simple enzymatic system can produce tandem repeats of monomers as short as 34 bp. In addition to suggesting possible origins for natural tandem repeats, these observations provide a facile means for constructing libraries of repeated motifs for use in "in vitro evolution" experiments designed to select molecules with defined biological or chemical properties. Abstract Natural genes and proteins often contain tandemly repeated sequence motifs that dramatically increase physiological specificity and activity. Publication types Research Support, Non-U. Gov't Research Support, U.

Proc natl acad sci u s a

Biocompatible gold nanoparticles designed to absorb light at wavelengths of high tissue transparency have been of particular interest for biomedical applications. The ability of such nanoparticles to convert absorbed near-infrared light to heat and induce highly localized hyperthermia has been shown to be highly effective for photothermal cancer therapy, resulting in cell death and tumor remission in a multitude of preclinical animal models. Here we report the initial results of a clinical trial in which laser-excited gold-silica nanoshells GSNs were used in combination with magnetic resonance-ultrasound fusion imaging to focally ablate low-intermediate-grade tumors within the prostate. The overall goal is to provide highly localized regional control of prostate cancer that also results in greatly reduced patient morbidity and improved functional outcomes. This pilot device study reports feasibility and safety data from 16 cases of patients diagnosed with low- or intermediate-risk localized prostate cancer. This treatment protocol appears to be feasible and safe in men with low- or intermediate-risk localized prostate cancer without serious complications or deleterious changes in genitourinary function.

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ProofWiki ID. X username. Page information. Tools What links here. Cookies help us deliver our services. The specific protein was then detected by either autoradiography, under UV light, or by the peroxidase reaction product, respectively. The New York Times. Mastodon address. For sodium dodecyl sulfate gels, the original band pattern was obtained with no loss of resolution, but the transfer was not quantitative. Reno, Nevada. In other projects. MiP and biochemistry.

As the area of small molecules interacting with RNA advances, general routes to provide bioactive compounds are needed as ligands can bind RNA avidly to sites that will not affect function. A non-oligonucleotide-containing compound was designed from sequence to target the precursor to oncogenic microRNA pre-miR for enzymatic destruction with selectivity that can exceed that for protein-targeted medicines.

Mir bel journal ID. X username. Add abstract. By using our services, you agree to our use of cookies. Earth-Science Reviews : For computational and data-enabled research, this has often been interpreted to mean making available the raw data from which results were generated, the computer code that generated the findings, and any additional information needed such as workflows and input parameters. Open access. In , PNAS issued an editorial stating its policy on publication of sensitive material in the life sciences. February Danish Bibliometric Research Indicator level. Academic journal of the National Academy of Sciences. Printable version. Dawid — Lawrence Bogorad — Nicholas R. October 13,