Smai cut site

Enzymes and Inhibitors. Restriction Enzymes. Catalog number: ER

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Smai cut site

Hi all, 1 Has anyone used smaI as a RE to cut a vector for blunt end cloning? So my situation now is I'm trying to cut a plasmid bp with smaI. I over digest overnight at room temp. Hope you guys can shed some light SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. I'd definately phosphatase my plasmid in order to prevent self ligation. I recently used Antarctic Phosphatase and it workes also very good. Good luck!!. Sma I has an isoschizomer which may be a better enzyme. If you still need the blunt end, you can just do a fill-in or chew back the overhang. Hi all, I juz started with cloning in pUC using SmaI to restrict digest for blunt ends and when i ran the gel got a linear plasmid but dint do dephosphorylation

Source: Serratia marcescens.

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Enzymes and Inhibitors. Restriction Enzymes. Catalog number: ER Related applications: Restriction Enzyme Cloning. Technical Support Customer Service.

Smai cut site

Listen to one of our scientific editorial team members read this article. Click here to access more audio articles or subscribe. Blunt ends can be easily created through PCR or enzymatic means. Blunt-end cloning is less efficient than traditional methods, and care should be given to avoid empty vector religation or insertion of fragments in both orientations. And do you know how to do both? Blunt and sticky might sound dull and dirty but knowing how these different cloning methods work is important when choosing which method to use. It is unlike sticky-end cloning, where both the insert and the vector contain single-stranded overhangs that complement each other.

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By creating an account, you confirm that you accept the Terms and Conditions and Privacy Policy. Sma I has an isoschizomer which may be a better enzyme. Forgot Password? Catalog number selected: R Europe Austria. Username Username not found. Password Password doesn't meet requirements. Storage Conditions. You've created a Promega. Resend Verification Email. Our records indicate that this email address is already registered. Pacific Asia Australia. Not for use in diagnostic procedures. Select All.

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Failure to heat the water will result in PEG that will take approximately 24 hours to dissolve. Add 4. Korea, Republic of. A password reset email has been sent to the primary email address associated with your account. Hi all, I juz started with cloning in pUC using SmaI to restrict digest for blunt ends and when i ran the gel got a linear plasmid but dint do dephosphorylation This product is available under our Early Access program - Learn More. BRL Technical Bulletin Please try again or contact Customer Service. Forum Index Home Live Discussion. Marketing Cookies We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media. For Research Use Only. Log In. Confirm New Password Passwords don't match. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.