Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In.

The SpectraViewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent labelled probes. Revvity Sites Globally Select your location. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, start by selecting the instrument from the Select Machine drop-down menu. The light source and filters for the chosen instrument will be displayed automatically. Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually. Light sources are input in nm format and filters as - Next, select the fluorophores of interest from the Add Fluorophore drop-down menu. You may add further fluorophores, light sources and filters at any point, or remove them by clicking X.

Spectra viewer

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time. Pre-loaded light sources and laser sets for microscopy and flow cytometry applications. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument. Generates a final report listing selected products, all the spectral profiles of fluorophores of interest, together with lasers and filters. Download and share fluorochrome spectrum alongside laser and filter configurations Generate, export, and share the final report.

To exit, click "cancel". If applicable, a list of similar compounds, as well as related categories, will be spectra viewer as well, spectra viewer. Adding Filters [ Show ] To add one or more filters, click "Filter" in the "Add" submenu on the left part of the screen.

.

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In. This can be useful for rotating through several overlapping spectra x. Add a spectrum to begin.

15.5 inches to cm

A vertical line will appear in the center of the graph display. Name Required. To predict the response of the fluorophores on the graph to an excitation laser, click "Predict Intensity" under the "Analyze" submenu. Navigation Menu. Toggling this will normalize the emission spectra to the selected light source line. Spectra Viewer and Experiment Design. Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually. Email Required. The emission graph profiles will change in real time as the line is dragged to various places on the horizontal axis, representing the intensity of response to an excitation source at that wavelength. To select all of a range of dyes, click the first desired choice, hold the SHIFT key while clicking the dye at the end of the desired range. Contact us Company. The email includes an automatically-assigned ID code which corresponds to your selections, which can be retrieved and loaded to the SpectraViewer by clicking Load and entering the ID code. A menu will appear below the graph display with common generic filters displayed on the left.

.

Light sources are input in nm format and filters as - Next, select the fluorophores of interest from the Add Fluorophore drop-down menu. The light source and filters for the chosen instrument will be displayed automatically. A - in a table cell represents no applicable spillover. Name Required. A vertical line will appear in the center of the graph display. To exit, click "cancel". Adjusting Graph Display [ Show ] If a selected compound is no longer of interest, click the checkbox to the right of the compound name as displayed on the bottom of the page to deselect it. To remove the filter from the workspace entirely, click the "x" to the right of the filter title. Then repeat the process with the bandwidth number. Click "Disable Intensity Prediction" to return to the default visual display. How to use this tool AAT Bioquest's interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats. Use the right-hand scroll bar to view the lower section of the tables if necessary. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. To view a known dye, scroll down the menu on the left and click to select. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument.